i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
Abstract We present a robust method called improved-Genome editing via A/C Delete Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation.The method generates mouse models containing single-base changes, kilobase-sized deletions, and knock-ins.The efficiency of i-GONAD is compar